2006

  2005     

    2004

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Selected Recent Publications

 

 

           2007

 

  • Kazanecki CC, Uzwiak DJ, Denhardt DT. (2007) Control of osteopontin signaling and function by post-translational phosphorylation and protein folding. J Cell Biochem. 102:912-24.

  • Kazanecki CC, Kowalski AJ, Ding T, Rittling SR, Denhardt DT. (2007) Characterization of anti-osteopontin monoclonal antibodies: Binding sensitivity to post-translational modifications. J Cell Biochem. 102:925-35.

  • Wang KX, Shi Y, Denhardt DT. (2007) Osteopontin regulates hindlimb-unloading-induced lymphoid organ atrophy and weight loss by modulating corticosteroid production. Proc Natl Acad Sci U S A. 104:14777-82.

  • Christensen B, Kazanecki CC, Petersen TE, Rittling SR, Denhardt DT, Sørensen ES. (2007) Cell type-specific post-translational modifications of mouse osteopontin are associated with different adhesive properties. J Biol Chem. 282:19463-72.

  • Kitamura M, Iwabuchi K, Kitaichi N, Kon S, Kitamei H, Namba K, Yoshida K, Denhardt DT, Rittling SR, Ohno S, Uede T, Onoé K. (2007) Osteopontin aggravates experimental autoimmune uveoretinitis in mice. J Immunol. 178:6567-72.

  • Hashimoto M, Sun D, Rittling SR, Denhardt DT, Young W. (2007) Osteopontin-deficient mice exhibit less inflammation, greater tissue damage, and impaired locomotor recovery from spinal cord injury compared with wild-type controls. J Neurosci. 27:3603-11.

  • Kavukcuoglu NB, Denhardt DT, Guzelsu N, Mann AB. (2007) Osteopontin deficiency and aging on nanomechanics of mouse bone. J Biomed Mater Res A. 83:136-44.

  • Wung JK, Perry G, Kowalski A, Harris PL, Bishop GM, Trivedi MA, Johnson SC, Smith MA, Denhardt DT, Atwood CS. (2007) Increased expression of the remodeling- and tumorigenic-associated factor osteopontin in pyramidal neurons of the Alzheimer's disease brain. Curr Alzheimer Res. 4:67-72.

  • Marsh BC, Kerr NC, Isles N, Denhardt DT, Wynick D. (2007) Osteopontin expression and function within the dorsal root ganglion. Neuroreport. 18:153-7.

  • Saika S, Shirai K, Yamanaka O, Miyazaki K, Okada Y, Kitano A, Flanders KC, Kon S, Uede T, Kao WW, Rittling SR, Denhardt DT, Ohnishi Y. (2007) Loss of osteopontin perturbs the epithelial-mesenchymal transition in an injured mouse lens epithelium. Lab Invest. 87:130-8.

  • Mori N, Majima T, Iwasaki N, Kon S, Miyakawa K, Kimura C, Tanaka K, Denhardt DT, Rittling S, Minami A, Uede T. (2007) The role of osteopontin in tendon tissue remodeling after denervation-induced mechanical stress deprivation. Matrix Biol. 26:42-53.

  • Hayashi C, Rittling S, Hayata T, Amagasa T, Denhardt D, Ezura Y, Nakashima K, Noda M. (2007) Serum osteopontin, an enhancer of tumor metastasis to bone, promotes B16 melanoma cell migration. J Cell Biochem. 101:979-86.

  • Kato N, Kitahara K, Rittling SR, Nakashima K, Denhardt DT, Kurosawa H, Ezura Y, Noda M. (2007) Osteopontin deficiency enhances anabolic action of EP4 agonist at a sub-optimal dose in bone. J Endocrinol. 193:171-82. 

  • Ishijima M, Tsuji K, Rittling SR, Yamashita T, Kurosawa H, Denhardt DT, Nifuji A, Ezura Y, Noda M. (2007) Osteopontin is required for mechanical stress-dependent signals to bone marrow cells. J Endocrinol. 193:235-43.

 

       2006

 

  • Da Silva AP, Pollett A, Rittling SR, Denhardt DT, Sodek J, Zohar R. (2006)Exacerbated tissue destruction in DSS-induced acute colitis of OPN-null mice is associated with downregulation of TNF-alpha expression and non-programmed cell death. J Cell Physiol. 208:629-39.

  • Ishijima M, Ezura Y, Tsuji K, Rittling SR, Kurosawa H, Denhardt DT, Emi M, Nifuji A, Noda M. (2006) Osteopontin is associated with nuclear factor kappaB gene expression during tail-suspension-induced bone loss. Exp Cell Res. 312:3075-83.

  • Lai CF, Seshadri V, Huang K, Shao JS, Cai J, Vattikuti R, Schumacher A, Loewy AP, Denhardt DT, Rittling SR, Towler DA. (2006)An osteopontin-NADPH oxidase signaling cascade promotes pro-matrix metalloproteinase 9 activation in aortic mesenchymal cells. Circ Res. 98:1479-89.

  • Schroeter M, Zickler P, Denhardt DT, Hartung HP, Jander S. (2006) Increased thalamic neurodegeneration following ischaemic cortical stroke in osteopontin-deficient mice. Brain. 129:1426-37.

  • Maeno Y, Nakazawa S, Yamamoto N, Shinzato M, Nagashima S, Tanaka K, Sasaki J, Rittling SR, Denhardt DT, Uede T, Taniguchi K. (2006) Osteopontin participates in Th1-mediated host resistance against nonlethal malaria parasite Plasmodium chabaudi chabaudi infection in mice. Infect Immun. 74:2423-7.

  • Koyama Y, Rittling SR, Tsuji K, Hino K, Salincarnboriboon R, Yano T, Taketani Y, Nifuji A, Denhardt DT, Noda M. (2006)Osteopontin deficiency suppresses high phosphate load-induced bone loss via specific modulation of osteoclasts. Endocrinology 147:3040-9

 

       2005

  • Porter JF, Sharma S, Wilson DL, Kappil MA, Hart RP, Denhardt DT. (2005) Tissue Inhibitor of Metalloproteinases-1 Stimulates Gene Expression in MDA-MB-435 Human Breast Cancer Cells by Means of its Ability to Inhibit Metalloproteinases. Breast Cancer Res Treat. 94:185-93.

  • Vernon HJ, Osborne C, Tzortzaki EG, Yang M, Chen J, Rittling SR, Denhardt DT, Buyske S, Bledsoe SB, Evan AP, Fairbanks L, Simmonds HA, Tischfield JA, Sahota A. (2005) Aprt/Opn double knockout mice: osteopontin is a modifier of kidney stone disease severity. Kidney Int. 68:938-47.

  • Zhu Y, Denhardt DT, Cao H, Sutphin PD, Koong AC, Giaccia AJ, Le QT. (2005)Hypoxia upregulates osteopontin expression in NIH-3T3 cells via a Ras-activated enhancer. Oncogene. 24:6555-63.

  • Kondo H, Nifuji A, Takeda S, Ezura Y, Rittling SR, Denhardt DT, Nakashima K, Karsenty G, Noda M. (2005) Unloading induces osteoblastic cell suppression and osteoclastic cell activation to lead to bone loss via sympathetic nervous system. J Biol Chem. 280:30192-200.

  • Nilsson SK, Johnston HM, Whitty GA, Williams B, Webb RJ, Denhardt DT, Bertoncello I, Bendall LJ, Simmons PJ, Haylock DN. (2005) Osteopontin, a key component of the hematopoietic stem cell niche and regulator of primitive hematopoietic progenitor cells. Blood. 106:1232-9.
  • Contractor T, Babiarz B, Kowalski AJ, Rittling SR, Sorensen ES, Denhardt DT. (2005) Osteoclasts resorb protein-free mineral (Osteologic discs) efficiently in the absence of osteopontin. In Vivo. 19:335-41.
  • Rollo EE, Hempson SJ, Bansal A, Tsao E, Habib I, Rittling SR, Denhardt DT, Mackow ER, Shaw RD. (2005) The cytokine osteopontin modulates the severity of rotavirus diarrhea. J Virol. 79:3509-16.


  • Denhardt D. (2005) Osteopontin expression correlates with melanoma invasion. J Invest Dermatol. 2005 May;124(5):xvi-xviii.


  • Khan SA, Cook AC, Kappil M, Günthert U, Chambers AF, Tuck AB, Denhardt DT (2006) Enhanced cell surface CD44 variant (v6, v9) expression by osteopontin in breast cancer epithelial cells facilitates tumor cell migration: Novel post-transcriptional, post-translational regulation. Clin. Expt. Metastasis (in press).

    •


       2004

  • Denhardt DT. (2004) The third international conference on osteopontin and related proteins, San Antonio, Texas, May 10-12, 2002.Calcif Tissue Int. 74:213-9.

  • Diao H, Kon S, Iwabuchi K, Kimura C, Morimoto J, Ito D, Segawa T, Maeda M, Hamuro J, Nakayama T, Taniguchi M, Yagita H, Van Kaer L, Onoe K, Denhardt D, Rittling S, Uede T. (2004) Osteopontin as a mediator of NKT cell function in T cell-mediated liver diseases. Immunity. 21:539-50.

  • Matsui Y, Jia N, Okamoto H, Kon S, Onozuka H, Akino M, Liu L, Morimoto J, Rittling SR, Denhardt D, Kitabatake A, Uede T. (2004) Role of osteopontin in cardiac fibrosis and remodeling in angiotensin II-induced cardiac hypertrophy. Hypertension 43:1195-201.

  • Morimoto J, Inobe M, Kimura C, Kon S, Diao H, Aoki M, Miyazaki T, Denhardt DT, Rittling S, Uede T. (2004) Osteopontin affects the persistence of beta-glucan-induced hepatic granuloma formation and tissue injury through two distinct mechanisms. Int Immunol. 16:477-88.

  • Ohyama Y, Nemoto H, Rittling S, Tsuji K, Amagasa T, Denhardt DT, Nifuji A, Noda M. (2004) Osteopontin-deficiency suppresses growth of B16 melanoma cells implanted in bone and osteoclastogenesis in co-cultures. J Bone Miner Res. 19:1706-11.

  • Weintraub AS, Lin X, Itskovich VV, Aguinaldo JG, Chaplin WF, Denhardt DT, Fayad ZA. (2004) Prenatal detection of embryo resorption in osteopontin-deficient mice using serial noninvasive magnetic resonance microscopy. Pediatr Res. 55:419-24.

  • Zhu B, Suzuki K, Goldberg HA, Rittling SR, Denhardt DT, McCulloch CA, Sodek J. (2004) Osteopontin modulates CD44-dependent chemotaxis of peritoneal macrophages through G-protein-coupled receptors: evidence of a role for an intracellular form of osteopontin. J Cell Physiol. 198:155-67.

 

       2003

  • Wong YF, Selvanayagam ZE, Wei N, Porter J, Vittal R, Hu R, Lin Y, Liao J,Shih JW, Cheung TH, Lo KW, Yim SF, Yip SK, Ngong DT, Siu N, Chan LK, Chan CS, Kong T, Kutlina E, McKinnon RD, Denhardt DT, Chin KV, Chung TK. (2003) Expression genomics of cervical cancer: molecular classification and prediction of radiotherapy response by DNA microarray. Clin Cancer Res. 9:5486-92

  • Morinobu M, Ishijima M, Rittling SR, Tsuji K, Yamamoto H, Nifuji A, Denhardt DT, Noda M. (2003) Osteopontin expression in osteoblasts and osteocytes during bone formation under mechanical stress in the calvarial suture in vivo. J Bone Miner Res. 18:1706-15.

  • Hampel DJ, Sansome C, Romanov VI, Kowalski AJ, Denhardt DT, Goligorsky MS. (2003) Osteopontin traffic in hypoxic renal epithelial cells. Nephron Exp Nephrol. 94:66-76.

  • Chellaiah MA, Biswas RS, Rittling SR, Denhardt DT, Hruska KA. (2003) Rho-dependent Rho kinase activation increases CD44 surface expression and bone resorption in osteoclasts. J Biol Chem. 278:29086-97.

  • Matsui Y, Rittling SR, Okamoto H, Inobe M, Jia N, Shimizu T, Akino M, Sugawara T, Morimoto J, Kimura C, Kon S, Denhardt D, Kitabatake A, Uede T. (2003) Osteopontin deficiency attenuates atherosclerosis in female apolipoprotein E-deficient mice. Arterioscler Thromb Vasc Biol. 23:1029-34.

  • Kitahara K, Ishijima M, Rittling SR, Tsuji K, Kurosawa H, Nifuji A, Denhardt DT, Noda M. (2003) Osteopontin deficiency induces parathyroid hormone enhancement of cortical bone formation. Endocrinology 144:2132-40.

  • Denhardt DT. (2003) Commentary re: Q-T Le et al, identification of osteopontin as a prognostic marker for head and neck squamous cell carcinomas. Clin Cancer Res. 9:31-2.

  • Chellaiah MA, Kizer N, Biswas R, Alvarez U, Strauss-Schoenberger J, Rifas L, Rittling SR, Denhardt DT, Hruska KA. (2003) Osteopontin deficiency produces osteoclast dysfunction due to reduced CD44 surface expression. Mol Biol Cell. 14:173-89.



       2002

  • Chabas, D.,Baranzini, S.E., Mitchell, D., Bernard, C.C.A., Rittling, S.R., Denhardt, D.T., Sobel, R.A., Lock, C. Karpuj, M, Pedotti, R., Heller, R. Oksenberg, J.R., and Steinman, L. (2001) Osteopontin Influences The Transition from Relapsing / Remitting to Progressive Autoimmune Demyelinating Disease. Science294:1731-1735.

  • Noda, M., and Denhardt, D. T. (2002) Osteopontin. In Principles of Bone Biology, 2nd ed. vol 1, 239-250. Academic Press.

  • Ishijima, M, Tsuji, K, Rittling, SR, Yamashita, T, Kurosawa, H, Denhardt, DT, Nifuji, A, Noda M. (2002) Resistance to unloading-induced three-dimensional bone loss in osteopontin-deficient mice. J Bone Mineral Res. 4:661-7.

  • D’Alonzo, R.C., Kowalski, A. J., Denhardt, D. T., Nickols, G. A., and Partridge, N. C. (2002) Regulation of collagenase-3 and osteocalcin gene expression by collagen and osteopontin in differentiating MC3T3-E1 cells. J. Biol. Chem. 277: 24788-24798.

  • Yumoto, K., Ishijima, M., Rittling, S.R., Tsuji, K., Tsuchiya, Y., Kon, S., Nifuji, A., Uede, T. Denhardt, D.T., and Noda, M. (2002) Ostopontin deficiency protects joints against destruction in anti-type II collagen antibody-induced arthritis in mice. Proc. Natl. Acad. Sci. USA 99:4556-4561.

  • Suzuki, K., Zhu, B., Rittling, S.R., Denhardt, D.T., Goldberg, H.A., McCulloch, C.A., Sodek, J. (2002) Colocalization of intracellular osteopontin with CD44 is associated with migration, cell fusion,
    and resorption in osteoclasts. J Bone Miner Res 17:1486-1497.



           2001

 


  • Asou, Y., Rittling, S.R., Yoshitake, H., Tsuji, K., Shinomiya, K., Nifuji, A., Denhardt, D.T., and Noda, M. (2001) Osteopontin facilitates angiogenesis, accumulation of osteoclasts, and resorption of ectopic bone. Endocrinology 142:1325-1332.
  • Attur, M.G., Dave, M.N., Stuchin, S., Kowalski, A.S., Steiner, G., Abramson, S.B., Denhardt, D.T., and Amin, A.R. (2001) Osteopontin: An intrinsic inhibitor of inflammation in cartilage. Arthritis and Rheumatism 44:578-584.
  • Nemoto, H., Rittling, S.R., Yoshitake, H., Furuya, K., Amagasa, T., Tsuji, K., Nifuji, A., Denhardt, D.T., Noda, M. (2001) Osteopontin deficiency reduces experimental tumor cell metastasis to bone and soft tissues. J. Bone Mineral Res. 16:652-659.
  • Ihara H, Denhardt DT, Furuya K, Yamashita T, Muguruma Y, Tsuji K, Hruska KA, Higashiomoto S, Nifuji A, Rittling SR, Noda M. (2001) Parathyroid hormone-induced bone resorption does not occur in the absence of osteopontin. J. Biol. Chem. 276:13065-13071.
  • Denhardt, D.T., Burger, E.H., Kazanecki C, Krishna, S., Semeins, C.M., and Klein-Nulend, J. (2001) Osteopontin-deficient bone cells are defective in their ability to produce NO in response to pulsatile fluid flow. Biochem. Biophys. Res. Comm. 288:448-453.


          2000


  • Ashkar, S., Weber, G.F., Panoutsakopoulou, .V, Sanchirico, M.E., Jansson, M., Zawaideh, S., Rittling, S.R., Denhardt, D.T., Glimcher, M.J., Cantor, H. (2000) Eta‑1 (osteopontin): an early component of type‑1 (cell‑mediated) immunity. Science 287, 86086‑4.
  • Denhardt, D.T. (2000) On the paradoxical ability of TIMPs either to inhibit or to promote the development and progression of the malignant phenotype.  In “Inhibitors of Metalloproteinases in Development and Disease”. Pp 137-151.  Edited by S.P. Hawkes, D.R. Edwards, and R. Khokha.  Harwood Academic Publ. Lausanne, Switzerland.
  • Tian, J.T., Sørensen, E.S., Butler. W.T., Lopez, C.A., Sy, M.-S., and Denhardt, D.T. (2000) Osteopontin and Hyaluronic Acid Inhibit NO Synthesis by RAW264.7 Cells Stimulated with Inflammatory Mediators: Collagen Blocks Inhibition by Osteopontin. Cytokine 12:450-457.
  • Bonvini, J.M., Schatzmann, U., Beck-Schimmer, B., Sun, L.K., Rittling, S.R., Denhardt, D.T., Hir, M.L., and Wuthrich, R.P. (2000) Lack of an in vivo function of osteopontin in experimental anti-GBM nephritis. J. Am. Soc. Nephrol. 11:1647-1655.

  • Wu, Y., Denhardt, D.T., and Rittling, S.R. (2000) Osteopontin expression is     required for full expression of the transformed phenotype by the Ras oncogene. Brit. J. Cancer. 83:156-173.



           1999


  • Yoshitake, H., Rittling, S.R., Denhardt, D.T., Noda, M. (1999) Osteopontin-deficient mice are resistant to ovariectomy-induced bone resorption. Proc. Natl. Acad. Sci. (US) 96:8156-8160.

  • Rittling, S.R., and Denhardt, D.T. (1999) Osteopontin function in pathology: Lessons from osteopontin-deficient mice. Exptl. Nephrology 7:103-113.



          1998

  • Rittling, S.R., Matsumoto, H.N., McKee, M.D., Nanci, A., An, X.-R., Novick, K., Kowalski, A., Noda, M., and Denhardt, D.T. (1998) Mice lacking osteopontin show normal development and bone structure but display altered osteoclast formation in vitro. Journal Bone Mineral Research 13:1101-1111.

  • Rajan, S.S. Lackland, H., Stein, S., and Denhardt, D.T. (1998) Presence of an N-terminal polyhistidine tag facilitates stable expression of an otherwise unstable N-terminal domain of mouse tissue inhibitor of metalloproteinase-1 in E. coli. Protein Expression and Purification 13:67-72 .

  • Rajan, S.S., Denhardt, D.T., Lackland, H., and Ludescher, R.D. (1998) Analysis of the conformational stability of the active domain of recombinant mouse TIMP-1 by intrinsic fluorescence. Biochem. Biophys. Res. Comm. 242:303-309.



           1996


  • Denhardt, D.T. (1996) Oncogene-initiated aberrant signaling engenders the metastatic phenotype: Synergistic transcription factor interactions are targets for cancer therapy. Crit. Revs. Oncogenesis 7:261-291.

  • Denhardt, D.T. (1996) Signal Transducing Protein Phosphorylation Cascades Mediated by RAS/RHO Proteins in the Mammalian Cell: The Potential for Multiplex Signalling. Biochemical Journal 318:729-747.

  • Rollo, E.E., Laskin, D.L., and Denhardt, D.T. (J. Leukocyte Biology 60, 397-404, 1996)Osteopontin inhibits nitric oxide production and cytotoxicity by activated RAW264.7 macrophages

  • Rollo, E.E., and Denhardt, D.T. (1996) Differential Effects of Osteopontin on the Cytotoxic Activity of Macrophages from Young and Old Mice. Immunology 88:642-647.

  • Walther, S.E., and Denhardt, D.T. (Cell Growth and Differentiation, 7, 1579-1588, 1996). Directed Mutagenesis Reveals that Two Histidines in Tissue Inhibitor of Metalloproteinase-are Each Essential for the Suppression of Cell Migration, Invasion and Tumorigenicity

  • Malyankar, U.M., Rittling, S.R., and Denhardt, D.T. (1996) Generation of Stable Mitogen Regulated Protein/Proliferin mRNA in Mouse Embryo Fibroblasts Transiently Transfected with Genomic Clones. J. Cellular Biochemistry 60:198-210.



           1995


  • Feng, B., Rollo, E.E., and Denhardt, D.T. (1995) Osteopontin (OPN) may Facilitate Metastasis by Protecting Cells from Macrophage NO-mediated Cytotoxicity: Evidence from Cell Lines Down-regulated for OPN Expression by a Targeted Ribozyme. Clin. Exptl. Metastasis 13:453-462.
  • Lopez, C.A., Olson, E.S., Adams, J.C., Mou, K., Denhardt, D.T., and Davis, R.L. (1995) Osteopontin Expression Detected in Adult Cochlea and Inner Ear Fluids. Hearing Research85:210-222.
  • Guo, X., Zhang, Y.P., Mitchell, D.A., Denhardt, D.T. and Chambers, A.F. (1995) Identification of a ras-Activated Enhancer in the Mouse Osteopontin Gene and its Interaction with a Putative ETS-related Transcription Factor Whose Activity Correlates with the Metastatic Potential of the Cell. Mol. Cell. Biol. 15:476-487.


          1994

  • Malyankar, U.M., Rittling, S.R., Connor, A., and Denhardt, D.T. (1994) The MRP/proliferin Transcript is Degraded in Primary Mouse Embryo Fibroblast but not 3T3 Nuclei: Altered RNA Processing Correlates with Immortalization. Proc. Natl. Acad. Sci. USA 91:335-339.

  • Hwang, S.-m., Lopez, C.A., Heck, D.E., Gardner, C.R., Laskin, D.L., Laskin, J.D., and Denhardt, D.T. (1994) Osteopontin Inhibits Induction of Nitric Oxide Synthase Gene Expression by Inflammatory Mediators in Mouse Kidney Epithelial Cells. J. Biol. Chem. 269:711-715.