Guofeng You Professor Rutgers University Department of Pharmaceutics Ernest Mario School of Pharmacy William Levine Hall Piscataway. NJ 08854 (732) 445-3831, Ext 218 FAX - 3134 gyou@rci.rutgers.edu

Molecular and cellular pharmacology. drug/toxin elimination. transporter

Drug transporters mediate the absorption, distribution, and excretion of a diverse array of clinically important drugs, including anti-HIV therapeutics, anti-tumor drugs, antibiotics, anti-hypertensives, and anti-inflammatories, and therefore are critical to the survival of the mammalian species. The long term goal of Dr. You's research is to elucidate the molecular and functional characteristics of these transporters, their implications in human physiology and diseases, and their applications to drug therapy. Currently, her research focuses on 1) the structure and function relationships of these transporters, and 2) their short-term and long-term regulatory mechanisms. Techniques in molecular biology, biochemistry, and biophysics are used to analyze the transport mechanisms. The knowledge gained from these studies will have significant impact on the future design of strategies aimed at maximizing therapeutic efficacy and minimizing toxicity, and will permit insight into the molecular, cellular, and clinical bases of renal, hepatic, neurological and fetal toxicity and disease.

Selected Publications

You, G. (2008) Membrane transporters in drug disposition. Pharmaceutical Research 25(2): 441-3

Zhou, F., Tanaka, K., and You, G. (2008) Comparison of the interaction of human organic anion transporter hOAT4 with PDZ proteins between kidney cells and placental cells.
Pharmaceutical Research 25(2):475-80

Zhou, F., Hong, M, and You, G. (2007) Regulation of human organic anion transporter 4 (hOAT4) by progesterone and protein kinase C in human placental BeWo cells. American Journal of Physiology 293(1):E57-61

Hong, M., Zhou, F., and You, G. (2007) Analysis of transmembrane domain 7 of the Human Organic Anion Transporter hOAT1. Journal of Pharmacology and Experimental Therapeutics 320(3):1209-15

Hong, M., Tanaka, K., Pan, Z., Ma, J., and You, G. (2007) Determination of the external loops and the cellular orientation of the amino- and the carboxyl termini of the human organic anion transporter hOAT1. Biochemical Journal 401(2):515-20

Zhou, F., and You, G. (2007) Molecular insights into the structure and function of organic anion transporters. Pharmaceutical Research 24(1):28-36

Xu, W., Tanaka, K., Sun, A., and You, G. (2006) The functional role of C terminus of human organic anion transporter hOAT1. Journal of Biological Chemistry 281:31178-83

Zhou, F., Illsley, N.P., and You, G. (2006) Functional characterization of a human organic anion transporter hOAT4 in placental BeWo cells. European Journal of Pharmaceutical Sciences 27(5):518-23

Hong, M., Xu, W., Yoshida, T., Tanaka, K., Zhou, F., Inouye, M., and You, G. (2005) Human organic anion transporter hOAT1 forms homooligomers. Journal of Biological Chemistry 280(37):32285-90

Zhou, F., Xu, W., Hong, M., Pan, Z., Sinko, P.J., Ma, J., and You, G. (2005) The role of N-linked glycosylation in protein folding, membrane targeting and substrate binding of human organic anion transporter hOAT4. Molecular Pharmacology 67(3):868-76

Hong, M., Zhou, F., and You, G. (2004) Critical amino acid residues in transmembrane domain 1 of the human organic anion transporter hOAT1. Journal of Biological Chemistry 279(30):31478-82

Zhou, F., Pan, Z., Ma, J., and You, G. (2004) Mutational analysis of histidine residues in the human organic anion transporter 4. Biochemical Journal 384:87-92

You, G. (2004) Towards an understanding of organic anion transporters: structure-function relationships. Medicinal Research Review 24:762-74

Zhou, F., Tanaka, K., Pan, Z., Ma, J., and You G. (2004) The role of glycine residues in the function of human organic anion transporter 4 (hOAT4). Molecular Pharmacology 65:1141-7.

Tanaka, K., Xu, W., Zhou, F., and You, G. (2004) Role of glycosylation in the organic anion transporter 1 (OAT1). Journal of Biological Chemistry 279:14961-6

You. G. (2004) The role of organic ion transporters in drug disposition: an update
Current Drug Metabolism 5:55-62.

Tanaka. K.. Zhou. F.. Kuze. K.. and You. G. (2004) Cysteine residues in the organic anion transporter mOAT1 Biochemical Journal 380:283-287

Zhou. F.. Tanaka. K.. Soares. M.J.. and You G. (2003) Characterization of an organic anion transport system in a placental cell line. American Journal of Physiology 285(5):E1103-9.

You. G. (2002) Structure. function. and regulation of drug transporters OAT. Medicinal Research Review 22(6):602-16.

You. G.. Kuze. K.. Kohanski. R. A.. Amsler. K.. and Handerson. S. (2000) Regulation of mOAT-mediated organic anion transport by okadaic acid and protein kinase C in LLC-PK1 cells. Journal of Biological Chemistry 275(14): 10278-10284.